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Progenitors in the postnatal cerebellar white matter are antigenically heterogeneous
Author(s) -
Milosevic Ana,
Goldman James E.
Publication year - 2002
Publication title -
journal of comparative neurology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.855
H-Index - 209
eISSN - 1096-9861
pISSN - 0021-9967
DOI - 10.1002/cne.10384
Subject(s) - biology , progenitor cell , olig2 , population , oligodendrocyte , progenitor , nestin , microbiology and biotechnology , stem cell , immunology , neural stem cell , neuroscience , central nervous system , myelin , demography , sociology
Progenitors that migrate through the white matter of the postnatal cerebellum give rise to cortical interneurons, astroglia, and oligodendroglia. To determine whether this progenitor population is heterogeneous with respect to specific lineage markers, we infected progenitors in vivo with a retrovirus encoding the green fluorescent protein on postnatal day 4/5 and labeled them in situ with various antibodies 2 days postviral injection: the neuronal marker was the transcription factor SOX1; early oligodendroglial markers were chondroitin sulfate proteoglycan antigen and platelet‐derived growth factor receptor‐α. Markers for astroglial progenitors were vimentin, nestin, zebrin II, and the astroglial‐specific glutamate transporter subtype GLAST. None of the progenitors was doubly labeled with any combination of markers characteristic for different cell lineages. Most progenitors were not labeled with any of the various combinations of antibodies used. Progenitors did not express markers characteristic for mature astroglia (GFAP), oligodendroglia (CNPase), or neurons (MAP2). Thus, although these progenitors are morphologically indistinguishable, a minority expresses markers of early neuronal or glial lineages, suggesting that they begin to differentiate during migration. J. Comp. Neurol. 452:192–203, 2002. © 2002 Wiley‐Liss, Inc.