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Immunolocalization of PICK1 in the ascending auditory pathways of the adult rat
Author(s) -
McInvale Andrew C.,
Staudinger Jeff,
Harlan Richard E.,
Garcia Meredith M.
Publication year - 2002
Publication title -
journal of comparative neurology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.855
H-Index - 209
eISSN - 1096-9861
pISSN - 0021-9967
DOI - 10.1002/cne.10328
Subject(s) - superior olivary complex , biology , neuroscience , neuropil , inferior colliculus , cochlear nucleus , medial geniculate body , auditory system , auditory cortex , lateral lemniscus , central nervous system , nucleus
Abstract Protein that interacts with C‐kinase α (PICK1) is a PDZ domain protein that interacts with many binding partners in the central nervous system (CNS), including activated protein kinase Cα and subunits of the AMPA subtype of glutamate receptor. Almost nothing is known about the anatomic distribution of PICK1 in the intact adult CNS. By using PICK1 antisera and peroxidase immunocytochemistry, we report on the distribution of PICK1 in the ascending pathways of the central auditory system of the adult rat. PICK1‐immunoreactivity (ir) was observed in many component nuclei of the central auditory system, including the dorsal cochlear nucleus, anteroventral cochlear nucleus, posteroventral cochlear nucleus, some divisions of the superior olivary complex, inferior colliculus, medial geniculate body, and primary auditory cortex. The general staining pattern for PICK1‐immunoreactivity was somatodendritic with scattered puncta in neuropil and somatodendritic regions. The distribution of PICK1 partially overlaps with PKCα and glutamate receptor subunits such as GluR2. These data suggest that PICK1 may function in the regulation of PKCα and GluR2 localization in components of the rat auditory system, which may be a fundamental mechanism of synaptic transmission and/or plasticity. J. Comp. Neurol. 450:382–394, 2002. © 2002 Wiley‐Liss, Inc.