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Differential expression of neuronal calcium sensor‐1 in the developing chick retina
Author(s) -
Bergmann Mathias,
Grabs Detlev,
Roder John,
Rager Günter,
Jeromin Andreas
Publication year - 2002
Publication title -
journal of comparative neurology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.855
H-Index - 209
eISSN - 1096-9861
pISSN - 0021-9967
DOI - 10.1002/cne.10302
Subject(s) - ribbon synapse , synaptogenesis , biology , retina , postsynaptic potential , microbiology and biotechnology , synaptic vesicle , neuroscience , outer plexiform layer , inner plexiform layer , calbindin , immunostaining , calcium binding protein , neurotransmission , ultrastructure , colocalization , anatomy , calcium , vesicle , immunohistochemistry , receptor , biochemistry , medicine , membrane , immunology
Neuronal calcium sensor‐1 (NCS‐1) is a Ca 2+ binding protein that has been implicated in the regulation of neurotransmission and synaptogenesis. In this study we investigated the developmental expression and localization of NCS‐1 in the chick retina. Single‐ and double‐labeling experiments with three‐dimensional reconstruction as well as ultrastructural data of the distribution of NCS‐1 suggest that this protein is also involved in axonal process outgrowth. We found an early expression of NCS‐1 in ganglion cells and their axons, in amacrine, and in horizontal cells, whereas photoreceptors were immunonegative at embryonic stages. In the early posthatching days we found strong immunostaining for NCS‐1 in horizontal cells and their processes in the outer plexiform layer. In contrast, synaptic vesicle protein 2 (SV2) was prominent only in photoreceptor synaptic terminals. Ultrastructural analysis confirmed that NCS‐1 was localized postsynaptically in horizontal cell processes, whereas presynaptic terminals were immunonegative. However, at late posthatching days we observed that photoreceptor ribbon synapses (from rods and/or cones) also expressed NCS‐1. Thus the results support the notion that NCS‐1 is involved in neuronal process outgrowth and is localized in pre‐ and postsynaptic compartments including mature photoreceptor synapses. J. Comp. Neurol. 449:231–240, 2002. © 2002 Wiley‐Liss, Inc.