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Use of FTA cards for the storage of breast carcinoma nucleic acid on fine‐needle aspiration samples
Author(s) -
Peluso Anna Lucia,
Cascone Anna Maria,
Lucchese Lucrezia,
Cozzolino Immacolata,
Ieni Antonio,
Mignogna Chiara,
Pepe Stefano,
Zeppa Pio
Publication year - 2015
Publication title -
cancer cytopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.29
H-Index - 57
eISSN - 1934-6638
pISSN - 1934-662X
DOI - 10.1002/cncy.21577
Subject(s) - medicine , fine needle aspiration , nucleic acid , pathology , biopsy , genetics , biology
BACKGROUND The preservation and storage of nucleic acids is important for DNA molecular techniques. The material obtained by fine‐needle aspiration (FNA) is often scanty and can not be wasted. FTA cards are filter papers that immobilize and stabilize nucleic acids and can be stored at room temperature. The current study evaluated whether nucleic acids of breast carcinoma cells, obtained by FNA in a clinical setting, may be collected, stored, and preserved on FTA cards. METHODS Thirty breast carcinoma, 5 non‐Hodgkin lymphoma (NHL), and 5 benign reactive lymph node (RLN) cell samples obtained by FNA were stored at −80°C and on FTA cards. DNA extraction and polymerase chain reaction were performed on cells at −80°C and on 2 punched disks of FTA cards. Fifty nanograms of extracted DNA from both sample types were used to amplify the Janus Kinase 2 ( JAK2 ) gene. RESULTS The mean value of DNA extracted from breast carcinoma cells was 28.19 ng/µL for that stored at −80°C and 3.28 ng/µL for that stored on FTA cards. Agarose gel analysis demonstrated expected bands of DNA in 29 cases (97%) with both methods. The mean value of DNA extracted from NHL and RLN samples was 37.54 ng/µL and 4.28 ng/µL, respectively, and agarose gel analysis demonstrated bands of high molecular weight DNA in both methods. Significant differences in DNA yield were found between storage at −80°C and FTA cards ( P <.0001), but no differences were detected between 260/280 nm ratios in breast carcinoma and NHL/RLN samples. CONCLUSIONS FTA cards can be conveniently used for the storage of breast carcinoma cells obtained by FNA, thus providing a reliable alternative to traditional methods. Cancer (Cancer Cytopathol) 2015;123:582–92. © 2015 American Cancer Society .