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Immunocytochemistry in effusion cytology
Author(s) -
Fetsch Patricia A.,
Abati Andrea
Publication year - 2001
Publication title -
cancer cytopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.052
H-Index - 304
eISSN - 1097-0142
pISSN - 0008-543X
DOI - 10.1002/cncr.9044
Subject(s) - medicine , cytology , immunocytochemistry , effusion , pathology , pleural effusion , cancer , differential diagnosis , cytopathology , etiology , radiology , surgery
Abstract BACKGROUND Cytology plays a pivotal role in the diagnosis of pleural effusions. In many cases, immunocytochemistry (ICC) is required to elucidate the etiology of the atypical cells. Effusions are samples that present unique problems for ICC. To date there is no standardization of ICC methods for effusions and cytology in general. METHODS The authors review the most commonly used cytologic preparations, fixatives, and antibodies used in effusion ICC. RESULTS Through the utilization of cell block preparations and a panel of antibodies appropriate for the differential diagnosis in question, ICC conditions utilized in surgical pathology can be most closely replicated. CONCLUSIONS ICC may provide reliable insights into various diagnostic dilemmas in effusion cytology, provided that laboratory standardization practices are followed. Cancer (Cancer Cytopathol) 2001;93:293–308. © 2001 American Cancer Society.