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High incidence of ras gene mutation in intrahepatic cholangiocarcinoma
Author(s) -
Tada Minoru,
Omata Masao,
Ohio Masao
Publication year - 1992
Publication title -
cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.052
H-Index - 304
eISSN - 1097-0142
pISSN - 0008-543X
DOI - 10.1002/cncr.2820690509
Subject(s) - gene , carcinogenesis , mutation , incidence (geometry) , intrahepatic cholangiocarcinoma , polymerase chain reaction , cancer research , gene mutation , medicine , genetics , biology , microbiology and biotechnology , gastroenterology , physics , optics
The DNA sequences around codons 12, 13, and 61 of the ras gene were analyzed by polymerase chain reaction and direct sequencing in 18 intrahepatic cholangiocar‐cinomas. The ras gene mutations were found in 9 of 18 (50%): 6 in K‐ ras codon 12, 1 in K‐ ras codon 13, 1 in K‐ ras codon 61, and 1 in N‐ ras codon 12. The incidence of mutations was higher in the hilar type of intrahepatic cholan‐giocarcinomas, especially when these tumors were large. The incidence and spectrum of the mutations were almost the same as those reported in colon cancers, possibly indicating similar etiologic agent(s) in the carcinogenesis of both cancers.