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MicroRNA‐200b reverses chemoresistance of docetaxel‐resistant human lung adenocarcinoma cells by targeting E2F3
Author(s) -
Feng Bing,
Wang Rui,
Song HaiZhu,
Chen LongBang
Publication year - 2011
Publication title -
cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.052
H-Index - 304
eISSN - 1097-0142
pISSN - 0008-543X
DOI - 10.1002/cncr.26560
Subject(s) - docetaxel , microrna , cancer research , ectopic expression , adenocarcinoma , gene knockdown , lung cancer , cancer , medicine , cell cycle , apoptosis , biology , oncology , cell culture , gene , genetics
Abstract BACKGROUND: MicroRNAs (miRNAs) have been identified as important posttranscriptional regulators involved in various biological and pathological processes of cells, but their association with tumor chemoresistance has not been fully understood. METHODS: We detected miRNA‐200b (miR‐200b) expression in different lung adenocarcinoma cell lines and then focused on its roles in regulation of docetaxel chemoresistance. We also identified E2F3 as a novel target of miR‐200b. RESULTS: Based on miRNA microarray data, miR‐200b was identified as the most down‐regulated miRNA in docetaxel‐resistant SPC‐A1/DTX cells compared with parental SPC‐A1 cells. Ectopic miR‐200b expression reversed docetaxel chemoresistance of lung adenocarcinoma cells through cell proliferation inhibition, apoptosis enhancement, and G 2 /M cell cycle arrest. In a nude mouse xenograft model, up‐regulation of miR‐200b significantly enhanced response of SPC‐A1/DTX cells to docetaxel. Luciferase reporters containing the 3′ untranslated region sequence of E2F3 messenger RNA were used to demonstrate that miR‐200b could directly target E2F3. Small interfering RNA–mediated E2F3 knockdown revealed similar effects as that of ectopic miR‐200b expression. Decreased miR‐200b expression was also detected in tumor tissues sampled from lung adenocarcinoma patients treated with docetaxel‐based chemotherapy and was proved to be correlated with high expression of E2F3, decreased sensitivity to docetaxel, and poor prognosis. CONCLUSIONS: Our results suggest that down‐regulation of miR‐200b could lead to E2F3 overexpression and in turn contribute to chemoresistance of lung adenocarcinoma cells to docetaxel. Cancer 2011. © 2011 American Cancer Society.