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Gene expression signatures in breast cancer distinguish phenotype characteristics, histologic subtypes, and tumor invasiveness
Author(s) -
Pedraza Vincente,
GomezCapilla Jose A.,
Escaramis Georgia,
Gomez Carolina,
Torné Pablo,
Rivera Jose M.,
Gil Angel,
Araque Patricia,
Olea Nicolas,
Estivill Xavier,
FárezVidal M. Esther
Publication year - 2009
Publication title -
cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.052
H-Index - 304
eISSN - 1097-0142
pISSN - 0008-543X
DOI - 10.1002/cncr.24805
Subject(s) - breast cancer , gene expression profiling , phenotype , gene , estrogen receptor , gene expression , cancer , biology , microarray analysis techniques , pathology , microarray , medicine , cancer research , genetics
BACKGROUND: The development of reliable gene expression profiling technology is having an increasing impact on the understanding of breast cancer biology. METHODS: In this study, microarray analysis was performed to establish gene signatures for different breast cancer phenotypes, to determine differentially expressed gene sequences at different stages of the disease, and to identify sequences with biologic significance for tumor progression. Samples were taken from patients before their treatment. After microarray analysis, the expression level of 153 selected genes was studied by real‐time quantitative polymerase chain reaction analysis. RESULTS: Several gene sequences were expressed differentially in tumor samples versus control samples and also were associated with different breast cancer phenotypes, estrogen receptor status, tumor histology, and grade of tumor differentiation. In lymph node‐negative tumors were identified a set of genes related to tumor differentiation grade. CONCLUSIONS: Several differentially expressed gene sequences were identified at different stages of breast cancer. Cancer 2010. © 2010 American Cancer Society.