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Detection of human papillomavirus infection in penile samples through liquid‐based cytology and polymerase chain reaction
Author(s) -
de Lima Rocha Maria Gabrielle,
Faria Fábio Lopes,
Souza Maria do Carmo M.,
Vago Annamaria Ravara,
Fernandes Ana Paula,
Fernandes Paula Ávila
Publication year - 2008
Publication title -
cancer cytopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.052
H-Index - 304
eISSN - 1097-0142
pISSN - 0008-543X
DOI - 10.1002/cncr.23947
Subject(s) - polymerase chain reaction , human papillomavirus , medicine , cytology , liquid based cytology , penile cancer , papillomaviridae , virology , pathology , cervical cancer , cancer , biology , genetics , gene
BACKGROUND. The human papillomavirus (HPV) is strongly related to cervical cancer and its precursor lesions. However, unlike in the case of women, there are limited data regarding HPV infection in men. Analysis of male HPV infection is frequently hindered by the lack of consistency in collection methods, sample adequacy, and low sensitivity of cytologic analysis. METHODS. The objective of the current study was to compare the results of liquid‐based cytology and HPV DNA testing through polymerase chain reaction in 99 penile samples collected from men presenting with condyloma acuminate or male partners of HPV‐infected women who had attended a public health service in the city of Belo Horizonte, Minas Gerais, Brazil. Classic and nonclassic cytomorphologic signs were adopted to evaluate the presence of HPV infections in penile smears. RESULTS. HPV DNA was detected in 93 (93.9%) of the 99 samples analyzed. Koilocytosis was detected in 1 smear and nonclassic signs were detected in 23 smears, 22 of which were found to be positive for HPV DNA. CONCLUSIONS. The cytopathologic detection of HPV infection in penile samples collected for liquid‐based cytology is low, even when cytologic nonclassic signs are applied, and does not appear to improve the diagnosis of HPV infection in men. Cancer (Cancer Cytopathol) 2008. © 2008 American Cancer Society.