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Tumor lymphangiogenesis correlates with lymph node metastasis and clinicopathologic parameters in oral squamous cell carcinoma
Author(s) -
Miyahara Mayumi,
Tanuma Junichi,
Sugihara Kazumasa,
Semba Ichiro
Publication year - 2007
Publication title -
cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.052
H-Index - 304
eISSN - 1097-0142
pISSN - 0008-543X
DOI - 10.1002/cncr.22900
Subject(s) - lymphangiogenesis , medicine , lymphatic vessel , pathology , immunohistochemistry , vascular endothelial growth factor c , lymphatic system , metastasis , lymph node , carcinoma , cancer , vascular endothelial growth factor , cancer research , vascular endothelial growth factor a , vegf receptors
BACKGROUND. Lymphatic vessel density (LVD) and microvessel density (MVD) are important parameters for assessing the malignant potential of tumors and patient survival. In this report, the authors defined LVD as the density of D2‐40‐positive lymphatic vessels and MVD as the density of CD105‐positive microvessels per unit area of tissue. It was reported previously that vascular endothelial growth factor C (VEGF‐C) is a major modulator of LVD and MVD. The objectives of this study were to clarify the clinical and prognostic significance of both LVD and MVD in oral squamous cell carcinoma (OSCC) and to elucidate the lymphangiogenic and angiogenic activities of VEGF‐C in cancer tissues. METHODS. In total, 110 OSCC tissue samples were evaluated for LVD, MVD, and expression of VEGF‐C using immunohistochemistry. Correlations among these parameters and clinicopathologic factors were examined. RESULTS. LVD was significantly higher in tumors that had very high expression of VEGF‐C compared with tumors that had no/weak expression of VEGF‐C. LVD correlated well with lymph node metastasis ( P < .001). MVD was correlated significantly with positive lymph node metastasis ( P < .001) but not with VEGF‐C expression. In contrast, high expression of VEGF‐C was correlated significantly with advanced tumor status ( P = .041). Survival rates were lower in patients who had higher LVD ( P < .001), higher MVD ( P = .0028), and strong VEGF‐C expression ( P = .048). CONCLUSIONS. Lymphangiogenesis predominantly influenced metastasis‐free survival. The current results suggested that LVD is a more useful tool than MVD and VEGF‐C for deciding on therapeutic strategies in patients with OSCC. Cancer 2007. © 2007 American Cancer Society.