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Immunocytochemical detection using CDX2
Author(s) -
Kobayashi Masako,
Ueyama Yuji,
Nakanishi Hayao,
Ishida Hiroji,
Takahashi Emiko,
Nakamura Shigeo,
Koshikawa Takashi,
Yatabe Yasushi
Publication year - 2006
Publication title -
cancer cytopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.052
H-Index - 304
eISSN - 1097-0142
pISSN - 0008-543X
DOI - 10.1002/cncr.21840
Subject(s) - malignancy , ascites , medicine , pathology , immunostaining , cytology , staining , cdx2 , pancreas , immunohistochemistry , cancer , carcinoma , immunocytochemistry , biology , gene expression , biochemistry , gene , homeobox
BACKGROUND Although ascites cytology is important for therapeutic strategies, it is difficult to distinguish cancer infiltration from reactive mesothelial proliferation in some patients. In this study, the authors applied CDX2 immunocytochemistry to improve diagnostic accuracy. METHODS The authors examined the distribution of CDX2 expression in carcinoma specimens using paraffin‐embedded tissues from various organs from 549 cancer patients. CDX2 immunostaining was applied to the 116 ascites specimens. RESULTS CDX2 expression was detected in a restricted range of cancers, with the vast majority of them originating from the gastrointestinal tract and pancreas. When applied to ascites specimens, no positive reactions were detected in any of the 81 cytology‐negative and molecular genetic analysis‐negative specimens. By contrast, 28 of 35 specimens diagnosed as suspicious for malignancy or malignancy showed a positive reaction. Furthermore, the authors found that a nuclear‐positive reaction was easily evaluated, even with a high level of background staining, and single cancer cells in 10 6 normal cells could be detected. CONCLUSION Results suggest that CDX2 is a specific and sensitive marker to detect gastrointestinal and pancreatic malignancies in ascites cytology. Cancer (Cancer Cytopathol) 2006. © 2006 American Cancer Society.