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Differential expression of insulin‐like growth factor binding protein 1 and ferritin light polypeptide in gestational trophoblastic neoplasia
Author(s) -
Feng HuiChen,
Tsao SaiWah,
Ngan Hextan Y.S.,
Xue WeiCheng,
Chiu PuiMan,
Cheung Annie N.Y.
Publication year - 2005
Publication title -
cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.052
H-Index - 304
eISSN - 1097-0142
pISSN - 0008-543X
DOI - 10.1002/cncr.21483
Subject(s) - suppression subtractive hybridization , ferritin , insulin like growth factor binding protein , cancer research , complementary dna , immunohistochemistry , cancer , microarray , placenta , microbiology and biotechnology , microarray analysis techniques , biology , medicine , choriocarcinoma , growth factor , endocrinology , gene expression , gene , fetus , insulin like growth factor , cdna library , receptor , genetics , pregnancy
BACKGROUND Hydatidiform mole (HM), the most common type of gestational trophoblastic diseases, can be considered as placenta with abnormal chromosome composition with potential of malignant transformation. Few biologic markers can predict subsequent development of persistent gestational trophoblastic neoplasia (GTN) requiring chemotherapy. METHODS Suppression subtractive hybridization (SSH) combined with cDNA microarray was used to compare the differential expression pattern of HM that spontaneously regressed and that subsequently developed metastatic GTN. Tissue‐specific chips were constructed from the subtracted cDNA libraries, followed by cDNA microarray analysis. Verification by quantitative RNA analysis by real‐time polymerase chain reaction (PCR) and immunohistochemical analysis was performed in 23 genotyped complete HM. RESULTS Sixteen differentially expressed transcripts were identified. Quantitative RNA analysis confirmed down‐regulation of ferritin light polypeptide (FTL) ( P = 0.037) and insulin‐like growth factor binding protein 1 (IGFBP1) ( P = 0.037) in HM that subsequently developed GTN when compared with those HM that regressed. Immunohistochemical analysis further confirmed reduced IGFBP1 protein ( P = 0.03) expression in HM that developed GTN. CONCLUSIONS Findings showed that reduced expression of genes related to cell invasion and immunosuppression, especially FTL and IGFBP1, were associated with development of GTN, and this finding may provide a better understanding of the pathogenesis of GTN. The potential application of FTL and IGFBP1 in management of patients with HM should be explored. Cancer 2005. © 2005 American Cancer Society.