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Cytokine/cytokine receptor gene expression in childhood acute lymphoblastic leukemia
Author(s) -
Wu Shuling,
Geßner Reinhard,
von Stackelberg Arend,
Kirchner Renate,
Henze Guenter,
Seeger Karl
Publication year - 2005
Publication title -
cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.052
H-Index - 304
eISSN - 1097-0142
pISSN - 0008-543X
DOI - 10.1002/cncr.20869
Subject(s) - cytokine , lymphoblast , medicine , bone marrow , immunology , cytokine receptor , real time polymerase chain reaction , gene expression , receptor , leukemia , cancer research , gene , biology , cell culture , biochemistry , genetics
BACKGROUND Recent studies have shown that cytokines/cytokine receptors (C/CR) affect leukemic cell growth and survival. The goal of the current study was to investigate possible correlations between gene expression patterns of C/CR in leukemic cells, clinical features, and outcome in children with acute lymphoblastic leukemia (ALL) at first disease recurrence. METHODS Between January 1997 and December 2000, bone marrow (BM) samples were collected from 68 children with first ALL recurrence at diagnosis. These patients were enrolled in the ALL‐REZ 95–96 disease recurrence trials of the Berlin‐Frankurt‐Münster study group. C/CR gene expression (interleukin [IL]‐7, IL‐10, IL‐12p40, IL‐15, IL‐18, IL‐7Rα, IL‐10R1, IL‐15Rα, interferon‐gamma [IFN‐γ], vascular epithelial growth factor [VEGF], Flt1, and transforming growth factor‐beta) was quantified by real‐time reverse transcriptase‐polymerase chain reaction and correlated with protein expression by immunofluorescence. RESULTS In comparison with T‐lineage ALL specimens, expression of IL‐10, IFN‐γ, IL‐15Rα, and Flt1 was significantly higher in B‐cell precursor (BCP) ALL specimens ( P < 0.01). Among BCP ALL samples, gene expression of IL‐7Rα and Flt1 was higher in pre‐B than in common or pro‐B leukemic cells. Moreover, expression levels of VEGF, IL‐7Rα, IL‐10R1, and IL‐15Rα were lower in lymphoblasts of patients with a combined BM recurrence than in those with an isolated recurrence ( P < 0.05). Children with IL‐15Rα expression above the median level had a significantly better probability of event‐free survival (0.65 vs. 0.34, P = 0.04) and survival (0.71 vs. 0.37, P = 0.02) at 5 years. CONCLUSIONS Expression of distinct C/CR in ALL cells was associated with lineage commitment and differentiation of leukemic cells, as well as with prognosis. It remains to be evaluated whether these prognostic and biologic findings of distinct C/CR expression in leukemic cells also have therapeutical implications for future antileukemic strategies. Cancer 2005. © 2005 American Cancer Society.