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Matrix metalloproteinase inhibitor reversion‐inducing cysteine‐rich protein with Kazal motifs
Author(s) -
Span Paul N.,
Sweep C. G. J. Fred,
Manders Peggy,
Beex Louk V. A. M.,
Leppert David,
Lindberg Raija L. P.
Publication year - 2003
Publication title -
cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.052
H-Index - 304
eISSN - 1097-0142
pISSN - 0008-543X
DOI - 10.1002/cncr.11395
Subject(s) - reversion , phenocopy , medicine , hazard ratio , matrix metalloproteinase , angiogenesis , breast cancer , cancer research , cancer , confidence interval , oncology , gene , biology , phenotype , genetics
Abstract BACKGROUND The recently described re version‐inducing c ysteine‐rich protein with K azal motifs (RECK) inhibits membrane Type 1 matrix metalloproteinase (MMP‐14), MMP‐2, and MMP‐9 secretion and enzymatic activity. Its expression is essential for normal vasculogenesis. Down‐regulation of RECK has been implicated in tumor angiogenesis and progression. METHODS The authors assessed the prognostic value of RECK expression in tumor tissue specimens from 278 breast carcinoma patients with a median follow‐up time of 75 months (range, 2–169 months). RECK mRNA levels were measured by real‐time quantitative reverse transcriptase–polymerase chain reaction. RESULTS Expression levels of RECK were lower in tumor tissue specimens than in adjacent normal breast tissue specimens from 10 patients ( P = 0.028). No relevant associations of RECK with established clinicopathologic factors or treatment regimens were found. RECK expression predicted a longer recurrence‐free survival time (RFS; P = 0.037) at the optimal cutoff value (hazard ratio, 0.66; 95% confidence interval, 0.44–0.98). The 100 patients whose tumors exhibited low levels of RECK had a mean RFS time of 80.4 months and a 61.8% 5‐year RFS rate, whereas the 178 patients with tumors with high RECK expression had a mean RFS time of 91.2 months and a 73.0% 5‐year RFS rate. Multivariate Cox regression analysis showed that RECK expression maintained a significant independent prognostic value for RFS time ( P = 0.047). CONCLUSIONS These results are in agreement with the notion of RECK being an important tumor‐suppressor gene. Therefore, the possibility of applying RECK, a pharmaceutical mimetic, or drugs activating endogenous RECK expression, as possible therapeutic or preventive agents for breast carcinoma should be explored. Cancer 2003;97:2710–5. © 2003 American Cancer Society. DOI 10.1002/cncr.11395