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ThinPrep®‐processed fine‐needle samples of breast are effective material for RNA‐ and DNA‐based molecular diagnosis
Author(s) -
Tisserand Pascaline,
Fouquet Coralie,
Marck Véronique,
Mallard Christine,
Fabre Monique,
Vielh Philippe,
Soussi Thierry
Publication year - 2003
Publication title -
cancer cytopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.052
H-Index - 304
eISSN - 1097-0142
pISSN - 0008-543X
DOI - 10.1002/cncr.11258
Subject(s) - rna , complementary dna , dna , medicine , computational biology , gene , microbiology and biotechnology , biology , genetics
BACKGROUND Fine‐needle sampling is the least invasive method of in vivo breast carcinoma sampling and can provide material for breast carcinoma diagnosis. The aim of the current study was to assess the accuracy of molecular diagnosis techniques using fine‐needle sample (FNS) material stored in PreservCyt® (Cytyc Corp., Boxborough, MA). METHODS The p53 tumor suppressor gene was chosen as a model because it can be used for DNA, RNA, and protein analysis. Molecular analysis was performed using a yeast functional assay and DNA sequencing. p53 accumulation was evaluated by immunocytochemistry. RESULTS DNA and protein analysis indicated that samples stored for periods of several months, either at room temperature, 4 °C, or −20 °C, can be processed reliably. For RNA‐based diagnosis, samples were still intact after 5 months of storage in PreservCyt® at 4 °C. In addition, using FNS material that was stored for 16 months at 4 °C, the authors detected p53 mutations with either the functional assay for separating alleles in yeast (an RNA‐based functional assay) or direct cDNA sequencing. CONCLUSIONS Fine‐needle samples stored in PreservCyt® at 4 °C are very good material for molecular diagnosis techniques. In addition, it is feasible to adopt a strategy of storing excess FNS material to create cellular banks that will be invaluable for future gene studies. Cancer (Cancer Cytopathol) 2003;99:223–32. © 2003 American Cancer Society. DOI 10.1002/cncr.11258