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Detection of tumor specific gene expression in bone marrow and peripheral blood from patients with small cell lung carcinoma
Author(s) -
Shingyoji Masato,
Takiguchi Yuichi,
Watanabe Reiko,
Hiroshima Kenzo,
Motoori Ken,
Kurosu Katsushi,
Kasahara Yasunori,
Tanabe Nobuhiro,
Tatsumi Koichiro,
Kuriyama Takayuki
Publication year - 2003
Publication title -
cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.052
H-Index - 304
eISSN - 1097-0142
pISSN - 0008-543X
DOI - 10.1002/cncr.11108
Subject(s) - medicine , bone marrow , pathology , circulating tumor cell , lung cancer , metastasis , carcinoma , lung , peripheral blood cell , cancer , oncology
BACKGROUND Small cell lung carcinoma (SCLC) has the propensity to grow rapidly and metastasize extensively. Detection of micro‐dissemination of SCLC may have clinical relevance. For its detection, tumor‐specific gene expressions were examined in peripheral blood and bone marrow aspirate from patients with SCLC. METHODS Expression of prepro‐gastrin‐releasing peptide (preproGRP), neuromedin B receptor (NMB‐R) and gastrin‐releasing peptide receptor (GRP‐R) were examined by reverse transcriptase polymerase chain reaction (RT‐PCR) in peripheral blood and bone marrow aspirate from 40 untreated patients with SCLC. Control samples consisted of peripheral blood samples from 5 patients with nonsmall cell lung cancer (NSCLC) and 20 healthy volunteers. RESULTS Positive rates of preproGRP, NMB‐R, and GRP‐R in bone marrow aspirate of patients with SCLC were 23% (9/40), 8% (3/40), and 10% (4/40), respectively. Those rates in peripheral blood were 11% (4/38), 5% (2/38), and 29% (11/38), respectively. Although GRP‐R expression was detected in patients with NSCLC and in healthy volunteers, preproGRP and NMB‐R expressions were not detected in patients with NSCLC and in healthy volunteers. All three gene expressions in bone marrow were more frequently observed in patients with bone marrow metastasis, accessed by biopsy, than in patients without. PreproGRP gene expression in bone marrow was also more frequent in patients with bone metastasis, accessed by bone scintigram, than in patients without, and was related to poorer survival. CONCLUSIONS Micro‐dissemination of SCLC was detectable by RT‐PCR of preproGRP and NMB‐R, both specific for SCLC. These gene expressions in bone marrow may be related to disease extent and prognosis. Cancer 2003;97:1057–62. © 2003 American Cancer Society. DOI 10.1002/cncr.11108