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Matrix metalloproteinase‐26 is expressed in human endometrium but not in endometrial carcinoma
Author(s) -
Isaka Keiichi,
Nishi Hirotaka,
Nakai Hiromi,
Nakada Toshihide,
Feng Li Yin,
Ebihara Yoshiro,
Takayama Masaomi
Publication year - 2002
Publication title -
cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.052
H-Index - 304
eISSN - 1097-0142
pISSN - 0008-543X
DOI - 10.1002/cncr.11030
Subject(s) - stromal cell , matrilysin , immunohistochemistry , carcinoma , endometrium , pathology , reverse transcription polymerase chain reaction , matrix metalloproteinase , biology , messenger rna , epithelium , microbiology and biotechnology , cancer research , medicine , gene , endocrinology , biochemistry
BACKGROUND The human matrix metalloproteinase (MMP)‐26, also called matrilysin‐2 or endometase, has been isolated as a matrilysin (MMP‐7) homolog. Matrix metalloproteinase‐26 was expressed in tissue samples from the placenta and endometrial tumors and its expression may be related to the development of endometrial carcinomas. METHODS Total RNAs were isolated from 5 endometrial carcinoma cell lines, 36 normal endometrial tissue samples, 4 hyperplasia tissue samples, and from 24 endometrial carcinoma tissue samples. Reverse transcription‐polymerase chain reation (RT‐PCR) was performed to detect MMP‐26 mRNA expression. To identify MMP‐26 mRNA localization and protein expression, we performed in situ RT‐PCR and immunohistochemistry, respectively. RESULTS Reverse transcription‐polymerase chain reaction analysis revealed that MMP‐26 mRNA was expressed in 24 of 36 normal human endometrial tissue samples. However, MMP‐26 mRNA expression was not detected in endometrial carcinoma cell lines nor in endometrial carcinoma tissue samples except for one case. Western blot analysis showed similar results. In situ RT‐PCR analysis revealed that MMP‐26 expression was localized in the epithelial glandular cells but faint expression was observed in the stromal cells. Subsequently, we separated endometrial tissues into epithelial glandular and stromal cells. Using RT‐PCR, the purified epithelial glandular cells exhibited MMP‐26 mRNA expression but the purified stromal cells did not. Immunohistochemical analyses revealed that MMP‐26 protein expression is also limited to endometrial epithelial glandular cells but not to cancer cells. Therefore, MMP‐26 expression is limited to normal epithelial glandular cells. CONCLUSIONS We found a significant difference in MMP‐26 expression in normal and malignant endometrial tissue samples, although its function is still unknown. These data suggest that MMP‐26 may be a candidate for a new tumor marker for endometrial carcinomas. Cancer 2003;97:79–89. © 2003 American Cancer Society. DOI 10.1002/cncr.11030