Premium
Immunohistochemically detected hepatic micrometastases predict a high risk of intrahepatic recurrence after resection of colorectal carcinoma liver metastases
Author(s) -
Yokoyama Naoyuki,
Shirai Yoshio,
Ajioka Yoich,
Nagakura Shigenori,
Suda Takeyasu,
Hatakeyama Katsuyoshi
Publication year - 2002
Publication title -
cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.052
H-Index - 304
eISSN - 1097-0142
pISSN - 0008-543X
DOI - 10.1002/cncr.10422
Subject(s) - medicine , cytokeratin , micrometastasis , immunohistochemistry , metastasis , pathology , colorectal cancer , gastroenterology , intrahepatic cholangiocarcinoma , carcinoma , cancer
BACKGROUND Hepatic metastases from colorectal carcinoma frequently recur after resection and hepatic micrometastases most likely are important in the development of such recurrences. The objectives of the current study were to assess the feasibility of the immunohistochemical detection of hepatic micrometastases from colorectal carcinoma and to determine their clinical significance. METHODS Fifty‐three patients underwent curative hepatic resection for colorectal carcinoma metastases. Multiple tissue sections were cut from the advancing margin of the largest hepatic metastasis in each patient and were stained with an antibody against cytokeratin‐20 to detect hepatic micrometastases, which were defined as discrete microscopic cancerous lesions surrounding the dominant metastasis. RESULTS Normal hepatocytes and intrahepatic bile duct epithelia stained negative for cytokeratin‐20 in all patients, whereas the largest hepatic tumors stained positive in 46 patients (86.8%). Among the 46 patients with hepatic tumors that were positive for cytokeratin‐20, hepatic micrometastases were found immunohistochemically in 32 patients (69.6%). The presence of hepatic micrometastases was associated with a larger number of macroscopic hepatic metastases ( P = 0.047) and patients with hepatic micrometastases were found to demonstrate a higher probability of intrahepatic recurrence ( P = 0.003) compared with those patients without hepatic micrometastases. In addition, patients with hepatic micrometastases demonstrated a worse survival (10‐year survival rate of 21.9%) compared with those patients without hepatic micrometastases (10‐year survival rate of 64.3%) ( P = 0.017). CONCLUSIONS Immunohistochemical detection of hepatic micrometastases is feasible in patients with colorectal carcinoma liver metastases. Hepatic micrometastasis indicates widespread hepatic involvement and thus predicts an increased risk of intrahepatic recurrence after hepatic resection and a poorer patient prognosis. Cancer 2002;94:1642–7. © 2002 American Cancer Society. DOI 10.1002/cncr.10422