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Matrix metalloproteinase‐7 expression and biologic aggressiveness of cholangiocellular carcinoma
Author(s) -
Miwa Shiro,
Miyagawa Shinichi,
Soeda Junpei,
Kawasaki Seiji
Publication year - 2002
Publication title -
cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.052
H-Index - 304
eISSN - 1097-0142
pISSN - 0008-543X
DOI - 10.1002/cncr.10235
Subject(s) - immunostaining , medicine , matrix metalloproteinase , western blot , carcinoma , pathology , immunohistochemistry , oncology , biology , gene , biochemistry
Abstract BACKGROUND Matrix metalloproteinase‐7 (MMP‐7) recently has been reported to play a role in tumor cell invasion. The objective of the current study was to examine the expression of MMP‐7 in patients with cholangiocellular carcinoma. METHODS Twenty‐six patients underwent resection of cholangiocellular carcinoma, leaving no macroscopic evidence of residual tumor. Immunostaining was performed to evaluate the relation between MMP‐7 expression and clinicopathologic features and patient prognosis. The immunostaining pattern of the tumor cells for MMP‐7 was classified as negative (−) ( n = 9), positive (+) ( n = 11), or strongly positive (++) ( n = 6). Western blot analysis was performed to investigate the expression of active or latent forms in cancerous and noncancerous lesions in four patients. RESULTS The survival rates in patients with MMP‐7 expression judged to be (−), (+), and (++) were 75%, 80%, and 0%, respectively, at 1 year, and 47%, 24%, and 0%, respectively, at 3 years. The survival rate of MMP‐7 (++) patients was significantly lower than that of MMP‐7 (+) patients ( P = 0.003) and MMP (−) patients ( P = 0.008). At last follow‐up, 3 patients in the MMP‐7 (−) group had survived for > 5 years. Western blot analysis demonstrated that there were two types of cholangiocellular carcinoma: those producing both latent and active MMP‐7 and those producing only the latent form. CONCLUSIONS Although the results of the current study are based on a small number of patients, they suggest that MMP‐7 expression is a significant prognostic factor in patients with cholangiocellular carcinoma and that cholangiocellular carcinoma demonstrating strongly positive expression of MMP‐7 on immunostaining may have a higher malignant potential compared with that showing negative or positive expression of MMP‐7. Cancer 2002;94:428–34. © 2002 American Cancer Society.