Open AccessApplications of Mechanochemistry for the Synthesis of DNA on Ionic Liquid Supports
Author(s) -
Johnston Christopher,
Hardacre Christopher,
Migaud Marie E.
Publication year - 2021
Publication title -
chemistry ‐ methods
Language(s) - English
Resource type - Journals
ISSN - 2628-9725
DOI - 10.1002/cmtd.202100002
Subject(s) - mechanochemistry , phosphoramidite , ionic liquid , monomer , oligonucleotide , materials science , phase (matter) , combinatorial chemistry , chemistry , chemical engineering , nanotechnology , organic chemistry , dna , polymer , engineering , biochemistry , catalysis
The standard methodology for the synthesis of oligonucleotides is the solid‐phase method. Here, we report the first case of mechanochemistry being directly applied to synthesising a dinucleotide on a soluble support. While mechanochemistry eliminates the need to solubilise a supported oligomer in the solution phase for phosphoramidite coupling, the support still confers the benefit of being able to purify the product by precipitation or by simple liquid‐liquid extractions as needed. Furthermore, this study also explores the use of mechanochemistry in the initial functionalisation of the ionic liquid support with a nucleoside monomer.