Open AccessNMR methods for studying membrane‐active antimicrobial peptides
Author(s) -
Strandberg Erik,
Ulrich Anne S.
Publication year - 2004
Publication title -
concepts in magnetic resonance part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.229
H-Index - 49
eISSN - 1552-5023
pISSN - 1546-6086
DOI - 10.1002/cmr.a.20024
Subject(s) - antimicrobial peptides , antimicrobial , membrane , chemistry , solid state nuclear magnetic resonance , lipid bilayer , combinatorial chemistry , organic chemistry , nuclear magnetic resonance , biochemistry , physics
NMR is a versatile tool for studying interactions between antimicrobial peptides and lipid membranes. Different approaches using both liquid state and solid state NMR are outlined here, with an emphasis on solid state NMR methods, to study the structures of antimicrobial peptides in lipid bilayers as well as the effect of these peptides on model membranes. Different NMR techniques for observing both peptides and lipids are explained, including 2 H, 13 C, 15 N, and 19 F labels, or natural abundance 1 H, 13 C, or 31 P. Previous studies in the field are extensively reviewed in easily accessible tables. © 2004 Wiley Periodicals, Inc. Concepts Magn Reson 23A: 89–120, 2004.