Open Access64 CuS‐labeled nanoparticles: a new sentinel‐lymph‐node‐mapping agent for PET–CT and photoacoustic tomography
Author(s) -
Liu Qiufang,
Zhou Min,
Li Panli,
Ku Geng,
Huang Gang,
Li Chun,
Song Shaoli
Publication year - 2016
Publication title -
contrast media & molecular imaging
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.714
H-Index - 50
eISSN - 1555-4317
pISSN - 1555-4309
DOI - 10.1002/cmmi.1709
Subject(s) - sentinel lymph node , positron emission tomography , biodistribution , axillary lymph nodes , nuclear medicine , medicine , lymph , biopsy , lymph node , imaging agent , tomography , sentinel node , breast cancer , radiology , biomedical engineering , in vivo , cancer , pathology , biology , microbiology and biotechnology
Determining sentinel lymph node (SLN) status is critical to cancer staging and treatment decisions. Currently, in clinical practice, 99m Tc‐radiocolloid‐mediated planar scintigraphy and single‐photon emission computed tomography (SPECT) are used to guide the biopsy and resection of SLNs. Recently, an emerging technique that combines positron emission tomography (PET) and photoacoustic tomography (PAT; PET–PAT) may offer accurate information in detecting SLNs. Herein, we report a kind of 64 CuS‐labeled nanoparticle ( 64 CuS‐NP) for the detection of SLNs with PET–PAT. We subcutaneously injected 64 CuS‐NPs into the rats’ forepaw pads. After 24 h, the rats’ first draining axillary lymph nodes (i.e. the SLNs) could be clearly visualized with micro‐PET (μPET)–CT. Rats were sacrificed after μPET–CT imaging, their axillary lymph nodes were surgically identified, and then PAT was employed to discover 64 CuS‐NP‐avid SLNs, which were embedded inside tissues. Biodistribution, autoradiography, and copper staining analyses confirmed the SLNs’ high uptake of 64 CuS‐NPs. Our study indicates that 64 CuS‐NPs are a promising dual‐function agent for both PET–CT and PAT and could be used with multi‐modal imaging strategies such as PET–PAT to identify SLNs in a clinical setting. Copyright © 2016 John Wiley & Sons, Ltd.