Open AccessMagnetite loaded Polypeptide‐PLGA multifunctional microbubbles for dual‐mode US/MR imaging
Author(s) -
Sun Ying,
Zhu Yunkai,
Huang Can,
Li Rongxin,
Chen Yaqing,
Duan Yourong
Publication year - 2015
Publication title -
contrast media & molecular imaging
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.714
H-Index - 50
eISSN - 1555-4317
pISSN - 1555-4309
DOI - 10.1002/cmmi.1675
Subject(s) - superparamagnetism , materials science , plga , fourier transform infrared spectroscopy , scanning electron microscope , microbubbles , transmission electron microscopy , nuclear magnetic resonance , chemistry , nuclear chemistry , biomedical engineering , nanotechnology , nanoparticle , ultrasound , medicine , optics , magnetization , radiology , physics , quantum mechanics , magnetic field , composite material
Magnetite loaded Polypeptide‐PLGA multifunctional microbubbles (Fe 3 O 4 /Polypeptide‐PLGA MMBs) that show superparamagnetic properties were prepared by a modified double emulsion method and employed as imaging agent for dual‐mode Ultrasound/Magnetic resonance (US/MR) imaging of prostatic cancer. The successful synthesis of MMBs was determined by Fourier Transform Infrared Spectrometer (FTIR), X‐ray diffraction (XRD), Transmission Electron Microscope (TEM), Scanning Electron Microscope (SEM), Atomic Absorption Spectroscopy (AAS) and vibrating sample magnetometer (VSM). The as‐prepared MMBs had a diameter of 700 nm and were quite safe as confirmed by MTT assays. Prussian Blue Staining showed that targeted Fe 3 O 4 /Polypeptide‐PLGA MMBs enhanced the cellular uptake efficiency. In cell attachment study, adherence of MMBs was significantly higher to LNCaP cells compared with negative control PC3 cells. The in vitro results demonstrated that these MMBs could enhance both US and MR imaging of prostatic cancer. Copyright © 2015 John Wiley & Sons, Ltd.