Open AccessSuperparamagnetic iron oxide nanoparticles conjugated to a grass pollen allergen and an optical probe
Author(s) -
Herranz Fernando,
SchmidtWeber Carsten B.,
Shamji Mohamed H.,
Narkus Annemarie,
RuizCabello Jesus,
Vilar Ramon
Publication year - 2012
Publication title -
contrast media & molecular imaging
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.714
H-Index - 50
eISSN - 1555-4317
pISSN - 1555-4309
DOI - 10.1002/cmmi.1466
Subject(s) - superparamagnetism , bioconjugation , iron oxide nanoparticles , conjugated system , nanoparticle , conjugate , iron oxide , fluorophore , chemistry , maghemite , streptavidin , nanotechnology , materials science , biotin , fluorescence , biochemistry , organic chemistry , polymer , magnetization , optics , mathematical analysis , physics , mathematics , quantum mechanics , magnetic field
ABSTRACT In this study we report the development of a bioconjugate between superparamagnetic iron oxide nanoparticles and Phl p5a (one of the major allergens from grass pollen). The bioconjugate also contains an optical probe (Alexa647) conjugated to the nanoparticle via biotin–streptavidin association. We show that this conjugate has a range of features that makes it a very promising candidate to image the localization of this allergen in vivo : (a) upon conjugation to the iron oxide nanoparticles, the allergen retains its ability to interact with IgE antibodies; (b) the magnetic properties of the iron oxide core of this bioconjugate are suitable for MR imaging; and (c) Alexa647 fluorophore retains its emission properties once attached to the iron oxide nanoparticles, yielding a dual modality MRI‐optical probe. Copyright © 2012 John Wiley & Sons, Ltd.