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Toward a Home Test for COVID‐19 Diagnosis: DNA Machine for Amplification‐Free SARS‐CoV‐2 Detection in Clinical Samples
Author(s) -
ElDeeb Ahmed A.,
Zablotskaya Sofia S.,
Rubel Maria S.,
Nour Moustapha A. Y.,
Kozlovskaya Liubov I.,
Shtro Anna A.,
Komissarov Andrey B.,
Kolpashchikov Dmitry M.
Publication year - 2022
Publication title -
chemmedchem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.817
H-Index - 100
eISSN - 1860-7187
pISSN - 1860-7179
DOI - 10.1002/cmdc.202200382
Subject(s) - rna , dna , nucleic acid , virology , detection limit , recombinase polymerase amplification , biology , primer (cosmetics) , polymerase chain reaction , reverse transcriptase , microbiology and biotechnology , sense (electronics) , computational biology , gene , chemistry , biochemistry , chromatography , organic chemistry
Nucleic acid‐based detection of RNA viruses requires an annealing procedure to obtain RNA/probe or RNA/primer complexes for unwinding stable structures of folded viral RNA. In this study, we designed a protein‐enzyme‐free nano‐construction, named four‐armed DNA machine (4DNM), that requires neither an amplification stage nor a high‐temperature annealing step for SARS‐CoV‐2 detection. It uses a binary deoxyribozyme (BiDz) sensor incorporated in a DNA nanostructure equipped with a total of four RNA‐binding arms. Additional arms were found to improve the limit of detection at least 10‐fold. The sensor distinguished SARS‐CoV‐2 from other respiratory viruses and correctly identified five positive and six negative clinical samples verified by quantitative polymerase chain reaction (RT‐qPCR). The strategy reported here can be used for the detection of long natural RNA and can become a basis for a point‐of‐care or home diagnostic test.