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Interaction of a Triantennary Quinoline Glycoconjugate with the Asialoglycoprotein Receptor
Author(s) -
Palit Subhadeep,
Banerjee Sayanika,
Mahata Tridib,
Niyogi Sougata,
Das Tanusree,
Sova Mandi Chandra,
Chakrabarti Partha,
Dutta Sanjay
Publication year - 2021
Publication title -
chemmedchem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.817
H-Index - 100
eISSN - 1860-7187
pISSN - 1860-7179
DOI - 10.1002/cmdc.202100158
Subject(s) - asialoglycoprotein receptor , glycoconjugate , endocytosis , intracellular , quinoline , targeted drug delivery , cytotoxicity , chemistry , biochemistry , galactose , receptor , biology , drug , pharmacology , in vitro , hepatocyte , organic chemistry
Targeted intracellular delivery is an efficient strategy for developing therapeutics against cancer and other intracellular infections. Nonspecific drug delivery shows limited clinical applications owing to high dosage, cytotoxicity, nonspecific action, high cost, etc. Therefore, targeted delivery of less cytotoxic drug candidates to hepatocytes through ASGPR‐mediated endocytosis could be an efficient strategy to surmount the prevailing shortcomings. In the present work, the gene encoding ASGPR‐H1‐CRD was amplified from Huh7 cells, cloned into pET 11a vector, and the ASGPR‐H1‐CRD protein was expressed and purified from E. coli . A novel triantennary galactose‐conjugated quinoline derivative 4 was synthesized that demonstrates 17‐fold higher binding affinity to isolated ASGPR‐H1‐CRD protein receptor ( K d ∼54 μM) in comparison to D‐galactose ( K d ∼900 μM). Moreover, micro‐calorimetric studies for the interaction of glycoconjugate 4 with ASGPR protein on live hepatocytes showed notable thermal response in case of ASGPR‐containing Huh7 cells, in comparison to non‐ASGPR Chang cells. These results might serve as an approach towards targeted delivery of small glycoconjugates to hepatocytes.