Premium
Front Cover: Detection of HER2 + Breast Cancer Cells using Bioinspired DNA‐Based Signal Amplification (ChemMedChem 8/2020)
Author(s) -
Rafiee Sarah D.,
Kocabey Samet,
Mayer Michael,
List Jonathan,
Rüegg Curzio
Publication year - 2020
Publication title -
chemmedchem
Language(s) - English
Resource type - Reports
SCImago Journal Rank - 0.817
H-Index - 100
eISSN - 1860-7187
pISSN - 1860-7179
DOI - 10.1002/cmdc.202000181
Subject(s) - dna , trastuzumab , breast cancer , microbiology and biotechnology , cancer cell , chemistry , antibody , flow cytometry , fluorescence , front cover , cancer , cancer research , biophysics , biology , cover (algebra) , biochemistry , genetics , physics , optics , mechanical engineering , engineering
The Front Cover illustrates the sensitive detection of HER2+ breast cancer cells by a DNA signal amplification circuit. Circulating cancer cells (CTC), hallmarks of metastatic cancer, are difficult to detect due to infrequent occurrence. The signal of the HER2+ cell binding antibody Trastuzumab is enhanced via a DNA hybridization chain reaction. The reaction is initiated by an initiator DNA strand, coupled to the antibody. This sequence triggers the hybridization of fluorescent hairpins, forming strongly labeled DNA constructs and allowing CTC detection by flow cytometry. Artwork by Miguel Spuch‐Calvar. More information can be found in the Communication by Sarah D. Rafiee, Samet Kocabey, Michael Mayer, Jonathan List, and Curzio Rüegg..