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Achieving Biocompatible SABRE: An in vitro Cytotoxicity Study
Author(s) -
Manoharan Anand,
Rayner Peter J.,
Iali Wissam,
Burns Michael J.,
Perry V. Hugh,
Duckett Simon B.
Publication year - 2018
Publication title -
chemmedchem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.817
H-Index - 100
eISSN - 1860-7187
pISSN - 1860-7179
DOI - 10.1002/cmdc.201700725
Subject(s) - biocompatible material , cytotoxicity , in vivo , chemistry , catalysis , in vitro , biomedical engineering , organic chemistry , biochemistry , medicine , microbiology and biotechnology , biology
Production of a biocompatible hyperpolarized bolus for signal amplification by reversible exchange (SABRE) could open the door to simple clinical diagnosis via magnetic resonance imaging. Essential to successful progression to preclinical/clinical applications is the determination of the toxicology profile of the SABRE reaction mixture. Herein, we exemplify the cytotoxicity of the SABRE approach using in vitro cell assays. We conclude that the main cause of the observed toxicity is due to the SABRE catalyst. We therefore illustrate two catalyst removal methods: one involving deactivation and ion‐exchange chromatography, and the second using biphasic catalysis. These routes produce a bolus suitable for future in vivo study.