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Synthesis of α‐Branched Acyclic Nucleoside Phosphonates as Potential Inhibitors of Bacterial Adenylate Cyclases
Author(s) -
Frydrych Jan,
Skácel Jan,
Šmídková Markéta,
MertlíkováKaiserová Helena,
Dračínský Martin,
Gnanasekaran Ramachandran,
Lepšík Martin,
SotoVelasquez Monica,
Watts Val J.,
Janeba Zlatko
Publication year - 2018
Publication title -
chemmedchem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.817
H-Index - 100
eISSN - 1860-7187
pISSN - 1860-7179
DOI - 10.1002/cmdc.201700715
Subject(s) - adenylate kinase , cyclase , bordetella pertussis , chemistry , bacillus anthracis , moiety , biochemistry , nucleoside , stereochemistry , linker , enzyme , biology , bacteria , computer science , genetics , operating system
Inhibition of Bordetella pertussis adenylate cyclase toxin (ACT) and Bacillus anthracis edema factor (EF), key virulence factors with adenylate cyclase activity, represents a potential method for treating or preventing toxemia related to whooping cough and anthrax, respectively. Novel α‐branched acyclic nucleoside phosphonates (ANPs) having a hemiaminal ether moiety were synthesized as potential inhibitors of bacterial adenylate cyclases. ANPs prepared as bisamidates were not cytotoxic, but did not exhibit any profound activity (IC 50 >10 μ m ) toward ACT in J774A.1 macrophages. The apparent lack of activity of the bisamidates is speculated to be due to the inefficient formation of the biologically active species (ANPpp) in the cells. Conversely, two 5‐haloanthraniloyl‐substituted ANPs in the form of diphosphates were shown to be potent ACT and EF inhibitors with IC 50 values ranging from 55 to 362 n m .