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An Integrin‐Targeting RGDK‐Tagged Nanocarrier: Anticancer Efficacy of Loaded Curcumin
Author(s) -
Das Krishnendu,
Nimushakavi Sahithi,
Chaudhuri Arabinda,
Das Prasanta Kumar
Publication year - 2017
Publication title -
chemmedchem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.817
H-Index - 100
eISSN - 1860-7187
pISSN - 1860-7179
DOI - 10.1002/cmdc.201700085
Subject(s) - nanocarriers , curcumin , chemistry , flow cytometry , mtt assay , cancer cell , apoptosis , microbiology and biotechnology , drug delivery , biochemistry , biology , cancer , genetics , organic chemistry
Herein we report the design and development of α 5 β 1 integrin‐specific noncovalent RGDK–lipopeptide‐functionalized single‐walled carbon nanotubes (SWNTs) that selectively deliver the anticancer drug curcumin to tumor cells. RGDK tetrapeptide‐tagged amphiphiles were synthesized that efficiently disperse SWNTs with a suspension stability index of >80 % in cell culture media. 3‐(4,5‐dimethylthiazol‐2‐yl)‐2,5‐diphenyltetrazolium bromide (MTT)‐ and lactate dehydrogenase (LDH)‐based cell viability assays in tumor (B16F10 melanoma) and noncancerous (NIH3T3 mouse fibroblast) cells revealed the non‐cytotoxic nature of these RGDK–lipopeptide–SWNT conjugates. Cellular uptake experiments with monoclonal antibodies against α v β 3 , α v β 5 , and α 5 β 1 integrins showed that these SWNT nanovectors deliver their cargo (Cy3‐labeled oligonucleotides, Cy3‐oligo) to B16F10 cells selectively via α 5 β 1 integrin. Notably, the nanovectors failed to deliver the Cy3‐oligo to NIH3T3 cells. The RGDK–SWNT is capable of delivering the anticancer drug curcumin to B16F10 cells more efficiently than NIH3T3 cells, leading to selective killing of B16F10 cells. Results of Annexin V binding based flow cytometry experiments are consistent with selective killing of tumor cells through the late apoptotic pathway. Biodistribution studies in melanoma (B16F10)‐bearing C57BL/6J mice showed tumor‐selective accumulation of curcumin intravenously administered via RGDK–lipopeptide–SWNT nanovectors.