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Cell Surface Nano‐modulation for Non‐invasive in vivo Near‐IR Stem Cell Monitoring
Author(s) -
Shin Woo Jung,
Shin Seung Won,
Yuk Ji Soo,
Amornkitbamrung Lunjakorn,
Jang Min Su,
Song In Hyun,
Choi Soon Won,
Kang Insung,
Lee Jin Young,
Bae Hojae,
Kang KyungSun,
Um Soong Ho
Publication year - 2017
Publication title -
chemmedchem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.817
H-Index - 100
eISSN - 1860-7187
pISSN - 1860-7179
DOI - 10.1002/cmdc.201600428
Subject(s) - stem cell , cell , in vivo , regenerative medicine , nanotechnology , microbiology and biotechnology , materials science , chemistry , biology , biochemistry
A stem cell tracking system is in high demand for the determination of cell destinations and for the validation of cell therapeutic efficacy in regenerative transplantation. To date, near‐infrared (NIR) imaging technology has received considerable attention in cell behavior monitoring, owing to its patient compatibility, easy accessibility and cost effectiveness. Conventionally, in vivo cell tracking has been visualized by direct in‐cell staining with NIR, where it may be achieved by complicated genetic engineering. Such genetic amendment techniques have suffered from serious challenges, which can destroy a cell's metabolism and can accidentally incur unexpected carcinoma. Herein we demonstrate a novel cell nano‐modulation method for noninvasive stem cell monitoring. It is simply achieved by conjugating stem cells with lipid‐supported, NIR‐tagged, polymeric nanoparticles. These engineered cells, which are designated as NIR‐labeled light‐emitting stem cells (LESCs), maintain their biochemical functionality (i.e., differentiation, quantum efficacy, etc.) even after conjugation. LESCs were used for in situ stem cell monitoring at inoculation sites. It is speculated that the LESC technique could provide a new preparative methodology for in vivo cell tracking in advanced diagnostic medicine, where cell behavior is a critical issue.