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Near‐Infrared Intraoperative Chemiluminescence Imaging
Author(s) -
Büchel Gabriel E.,
Carney Brandon,
Shaffer Travis M.,
Tang Jun,
Austin Christine,
Arora Manish,
Zeglis Brian M.,
Grimm Jan,
Eppinger Jörg,
Reiner Thomas
Publication year - 2016
Publication title -
chemmedchem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.817
H-Index - 100
eISSN - 1860-7187
pISSN - 1860-7179
DOI - 10.1002/cmdc.201600301
Subject(s) - chemiluminescence , biomedical engineering , molecular imaging , detection limit , near infrared spectroscopy , computer science , in vivo , medicine , chemistry , optics , physics , microbiology and biotechnology , biology , chromatography , organic chemistry
Abstract Intraoperative imaging technologies recently entered the operating room, and their implementation is revolutionizing how physicians plan, monitor, and perform surgical interventions. In this work, we present a novel surgical imaging reporter system: intraoperative chemiluminescence imaging (ICI). To this end, we have leveraged the ability of a chemiluminescent metal complex to generate near‐infrared light upon exposure to an aqueous solution of Ce 4+ in the presence of reducing tissue or blood components. An optical camera spatially resolves the resulting photon flux. We describe the construction and application of a prototype imaging setup, which achieves a detection limit as low as 6.9 pmol cm −2 of the transition‐metal‐based ICI agent. As a proof of concept, we use ICI for the in vivo detection of our transition metal tracer following both systemic and subdermal injections. The very high signal‐to‐noise ratios make ICI an interesting candidate for the development of new intraoperative imaging technologies.