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Effect of Transition Metals on Polysialic Acid Structure and Functions
Author(s) -
Murthy Raghavendra Vasudeva,
Bharate Priya,
Gade Madhuri,
Sangabathuni Sivakoti,
Kikkeri Raghavendra
Publication year - 2016
Publication title -
chemmedchem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.817
H-Index - 100
eISSN - 1860-7187
pISSN - 1860-7179
DOI - 10.1002/cmdc.201600023
Subject(s) - polysialic acid , isothermal titration calorimetry , neurite , chemistry , neural cell adhesion molecule , biophysics , circular dichroism , transition metal , microbiology and biotechnology , biochemistry , cell adhesion , cell , biology , in vitro , catalysis
Polysialic acid (PSA) is one of the most abundant glycopolymer present in embryonic brain, and it is known to be involved in key roles such as plasticity in the central nervous system, cell adhesion, migration and localization of neurotrophins. However, in adult brain, its expression is quite low. The exception to this is in Alzheimer′s disease (AD) brain, where significantly increased levels of polysilylated neural cell adhesion molecule (PSA‐NCAM) have been reported. Here, we confirm the role of PSA as a metal chelator, allowing it to decrease cytotoxicity caused by high levels of transition metals, commonly found in AD brain, and as a regulator of cell behavior. UV‐visible (UV‐vis) and circular dichroism (CD) spectroscopy, atomic force microscopy (AFM), and isothermal titration calorimetry (ITC) techniques were used to investigate the assembly of PSA–metals complexes. These PSA–metal complexes exhibited less toxicity compared to free metal ions, and in particular, the PSA–Cu 2+ complex synergistically promoted neurite outgrowth in PC12 cells.