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Inside Cover: Site‐Directed RNA Editing with Antagomir Deaminases — A Tool to Study Protein and RNA Function (ChemMedChem 9/2014)
Author(s) -
Vogel Paul,
Stafforst Thorsten
Publication year - 2014
Publication title -
chemmedchem
Language(s) - English
Resource type - Reports
SCImago Journal Rank - 0.817
H-Index - 100
eISSN - 1860-7187
pISSN - 1860-7179
DOI - 10.1002/cmdc.201490033
Subject(s) - rna , computational biology , protein function , messenger rna , biology , stop codon , rna editing , chemistry , microbiology and biotechnology , biochemistry , gene
The inside‐front cover picture shows transcript repair by site‐directed A‐to‐I RNA editing inside mammalian cells. Lipofected antagomirs steer an engineered deaminase specifically to the mRNA substrate and activate cyan fluorescent protein (CFP) fluorescence by reprogramming a premature STOP codon to tryptophan. The tool is applicable in medicine and biology. The assembly strategy may serve as a general blue‐print in synthetic biology and nanobiotechnology. For more details, see the Concept by Paul Vogel and Thorsten Stafforst on p. 2021 ff.