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Endothelial Transcriptome in Response to Pharmacological Methyltransferase Inhibition
Author(s) -
Okabe Jun,
Fernandez Ana Z.,
Ziemann Mark,
Keating Samuel T.,
Balcerczyk Aneta,
ElOsta Assam
Publication year - 2014
Publication title -
chemmedchem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.817
H-Index - 100
eISSN - 1860-7187
pISSN - 1860-7179
DOI - 10.1002/cmdc.201402091
Subject(s) - methyltransferase , methylation , histone , histone methyltransferase , protein arginine methyltransferase 5 , ezh2 , gene expression , histone methylation , biology , transcriptome , acetylation , activator (genetics) , signal transduction , regulation of gene expression , epigenetics , arginine , microbiology and biotechnology , chemistry , biochemistry , dna methylation , gene , amino acid
The enzymatic activities of protein methyltransferases serve to write covalent modifications on histone and non‐histone proteins in the control of gene transcription. Here, we describe gene expression changes in human endothelial cells caused by treatment with methyltransferase inhibitors 7,7′‐carbonylbis (azanediyl) bis(4‐hydroxynaphthalene‐2 ‐sulfonic acid (AMI‐1) and disodium‐2‐(2,4,5,7‐ tetrabromo‐3‐oxido‐6‐oxoxanthen‐9‐yl) benzoate trihydrate (AMI‐5). Deep sequencing of mRNA indicated robust change on transcription following AMI‐5 treatment compared with AMI‐1. Functional annotation analysis revealed that both compounds suppress the expression of genes associated with translational regulation, suggesting arginine methylation by protein arginine methyltransferases (PRMTs) could be associated with regulation of this pathway. Interestingly, AMI‐5 but not AMI‐1 was found to decrease methylation of H3 histones at lysine 4 and down‐regulate gene expression associated with interleukin‐6 (IL‐6) and activator protein‐1 (AP‐1) signaling pathways. These results imply that inhibition of protein methylation by AMI‐1 and AMI‐5 can differentially regulate specific pathways with potential to interrupt pathological signaling in the vascular endothelium.