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Gyrase ATPase Domain as an Antitubercular Drug Discovery Platform: Structure‐Based Design and Lead Optimization of Nitrothiazolyl Carboxamide Analogues
Author(s) -
Jeankumar Variam Ullas,
Renuka Janupally,
Kotagiri Sonali,
Saxena Shalini,
Kakan Shruti Singh,
Sridevi Jonnalagadda Padma,
Yellanki Swapna,
Kulkarni Pushkar,
Yogeeswari Perumal,
Sriram Dharmarajan
Publication year - 2014
Publication title -
chemmedchem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.817
H-Index - 100
eISSN - 1860-7187
pISSN - 1860-7179
DOI - 10.1002/cmdc.201402035
Subject(s) - dna gyrase , drug discovery , carboxamide , lead compound , computational biology , lead (geology) , chemistry , virtual screening , atpase , drug , pharmacology , combinatorial chemistry , stereochemistry , biology , biochemistry , enzyme , in vitro , gene , escherichia coli , paleontology
In this study, we explored the pharmaceutically underexploited mycobacterial gyrase ATPase (GyrB) domain as a template for a structure‐based virtual screening of our in‐house (BITS Pilani) compound collection to discover new inhibitors targeting Mycobacterium tuberculosis ( M.tb. ) The hit identified was further customized by using a combination of molecular docking and medicinal chemistry strategies to obtain an optimized analogue displaying considerable in vitro enzyme efficacy and bactericidal properties against the M.tb. H 37 Rv strain. The binding affinity of the ligand toward the GyrB domain was reascertained by differential scanning fluorimetry experiments. Further evaluation of the hERG toxicity (a major limitation among the previously reported N‐linked aminopiperidine analogues) indicated these molecules to be completely devoid of cardiotoxicity, a significant achievement within this class.