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High‐Throughput Screening of Metal‐ N ‐Heterocyclic Carbene Complexes against Biofilm Formation by Pathogenic Bacteria
Author(s) -
Bernardi Thierry,
Badel Stéphanie,
Mayer Pascal,
Groelly Jérome,
de Frémont Pierre,
Jacques Béatrice,
Braunstein Pierre,
Teyssot MarieLaure,
Gaulier Christelle,
Cisnetti Federico,
Gautier Arnaud,
Roland Sylvain
Publication year - 2014
Publication title -
chemmedchem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.817
H-Index - 100
eISSN - 1860-7187
pISSN - 1860-7179
DOI - 10.1002/cmdc.201402012
Subject(s) - biofilm , carbene , bacteria , chemistry , escherichia coli , metal , pseudomonas aeruginosa , microbiology and biotechnology , ligand (biochemistry) , pathogenic bacteria , high throughput screening , biology , organic chemistry , biochemistry , catalysis , gene , receptor , genetics
A set of molecules including a majority of metal‐ N ‐heterocyclic carbene (NHC) complexes (metal=Ag, Cu, and Au) and azolium salts were evaluated by high‐throughput screening of their activity against biofilm formation associated with pathogenic bacteria. The anti‐planktonic effects were compared in parallel. Representative biofilm‐forming strains of various genera were selected ( Listeria , Pseudomonas , Staphylococcus , and Escherichia ). All the compounds were tested at 1 mg L −1 by using the BioFilm Ring Test. An information score (IS, sum of the activities) and an activity score (AS, difference between anti‐biofilm and anti‐planktonic activity) were determined from normalized experimental values to classify the most active molecules against the panel of bacterial strains. With this method we identified lipophilic Ag I and Cu I complexes possessing aromatic groups on the NHC ligand as the most efficient at inhibiting biofilm formation.