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A Chemical Tool for In Vitro and In Vivo Precipitation of Lysine Methyltransferase G9a
Author(s) -
Konze Kyle D.,
Pattenden Samantha G.,
Liu Feng,
BarsyteLovejoy Dalia,
Li Fengling,
Simon Jeremy M.,
Davis Ian J.,
Vedadi Masoud,
Jin Jian
Publication year - 2014
Publication title -
chemmedchem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.817
H-Index - 100
eISSN - 1860-7187
pISSN - 1860-7179
DOI - 10.1002/cmdc.201300450
Subject(s) - in vivo , in vitro , lysine , methyltransferase , chemistry , biochemistry , computational biology , microbiology and biotechnology , biology , methylation , amino acid , dna
Here we report the design, synthesis, and biochemical characterization of a new chemical tool, UNC0965. UNC0965 is a biotinylated version of our previously reported G9a chemical probe, UNC0638. Importantly, UNC0965 maintains high in vitro potency and is cell penetrant. The biotinylated tag of UNC0965 enables “chemiprecipitation” of G9a from whole cell lysates. Further, the cell penetrance of UNC0965 allowed us to explore the localization of G9a on chromatin both in vitro and in vivo through chemical inhibitor‐based chromatin immunoprecipitation (chem‐ChIP).