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Cover Picture: Fluorogenic Peptide‐Based Substrates for Monitoring Thrombin Activity (ChemMedChem 4/2012)
Author(s) -
van Berkel Sander S.,
van der Lee Bas,
van Delft Floris L.,
Wagenvoord Rob,
Hemker H. Coenraad,
Rutjes Floris P. J. T.
Publication year - 2012
Publication title -
chemmedchem
Language(s) - English
Resource type - Reports
SCImago Journal Rank - 0.817
H-Index - 100
eISSN - 1860-7187
pISSN - 1860-7179
DOI - 10.1002/cmdc.201290012
Subject(s) - cover (algebra) , thrombin generation , peptide , thrombin , chemistry , combinatorial chemistry , nanotechnology , biochemistry , materials science , medicine , engineering , platelet , mechanical engineering
The cover picture shows thrombin‐specific substrates bound in the active site of thrombin. In the background, the intact fluorogenic conjugate (shown in yellow) of thrombin‐specific tripeptide H‐Gly‐Gly‐Arg and 7‐amino‐4‐methylcoumarin (AMC) bound to thrombin is displayed. Prior to thrombin‐mediate hydrolysis, this substrate displays only weak fluorescence. Upon cleavage of the ArgAMC bond, strongly fluorescent AMC (shown in bright blue) is liberated. Quantification of the increase in fluorescence over time provides a direct measure for the amount of thrombin present in plasma. Moreover, from these data, the specific thrombin activity can be calculated, which is a direct measure of the coagulability of a clotting sample. These substrates have potential application in microfluidic devices for point‐of‐care tests, allowing the rapid diagnosis of blood or plasma samples. For more details, see the Full Paper by Floris P. J. T. Rutjes et al. on p. 606 ff.