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Gadolinium‐ and Manganite‐Based Contrast Agents with Fluorescent Probes for Both Magnetic Resonance and Fluorescence Imaging of Pancreatic Islets: A Comparative Study
Author(s) -
Berkova Zuzana,
Jirak Daniel,
Zacharovova Klara,
Lukes Ivan,
Kotkova Zuzana,
Kotek Jan,
Kacenka Michal,
Kaman Ondrej,
Rehor Ivan,
Hajek Milan,
Saudek Frantisek
Publication year - 2013
Publication title -
chemmedchem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.817
H-Index - 100
eISSN - 1860-7187
pISSN - 1860-7179
DOI - 10.1002/cmdc.201200439
Subject(s) - in vivo , pancreatic islets , gadolinium , fluorescence , magnetic resonance imaging , islet , in vitro , chemistry , rhodamine , nuclear magnetic resonance , pancreas , pathology , biophysics , medicine , biology , biochemistry , endocrinology , radiology , diabetes mellitus , optics , physics , microbiology and biotechnology , organic chemistry
Three magnetic resonance (MR)/fluorescence imaging probes were tested for visualization, cellular distribution, and survival of labeled pancreatic islets in vitro and following transplantation. As T 1 contrast agents (CAs), gadolinium(III) complexes linked to β‐cyclodextrin (Gd‐F‐βCD) or bound to titanium dioxide (TiO 2 @RhdGd) were tested. As a T 2 CA, perovskite manganite nanoparticles (LSMO@siF@si) were examined. Fluorescein or rhodamine was incorporated as a fluorescent marker in all probes. Islets labeled with gadolinium(III) CAs were visible as hyperintense spots on MR in vitro, but detection in vivo was inconclusive. Islets labeled with LSMO@siF@si CA were clearly visible as hypointense spots or areas on MR scans in vitro as well as in vivo. All CAs were detected inside the islet cells by fluorescence. Although the vitality and function of the labeled islets was not impaired by any of the tested CAs, results indicate that LSMO@siF@si CA is a superior marker for islet labeling, as it provides better contrast enhancement within a shorter scan time.