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Identification of LasR Ligands through a Virtual Screening Approach
Author(s) -
Skovstrup Søren,
Le Quement Sebastian Thordal,
Hansen Thomas,
Jakobsen Tim Holm,
Harmsen Morten,
TolkerNielsen Tim,
Nielsen Thomas E.,
Givskov Michael,
Taboureau Olivier
Publication year - 2013
Publication title -
chemmedchem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.817
H-Index - 100
eISSN - 1860-7187
pISSN - 1860-7179
DOI - 10.1002/cmdc.201200434
Subject(s) - virtual screening , quorum sensing , pharmacophore , pseudomonas aeruginosa , computational biology , homoserine , transcription factor , chemistry , biology , microbiology and biotechnology , biochemistry , bacteria , genetics , biofilm , gene
With the widespread occurrence of bacterial resistance to antibiotics, the development of new strategies beyond conventional treatments is a pursuit taken by public health institutions worldwide. LasR, a transcription factor that controls quorum sensing in Pseudomonas aeruginosa , has emerged as an attractive therapeutic target for the next generation of antimicrobial agents. In the present study, a virtual screening workflow combining pharmacophore‐ and structure‐based approaches was used to identify new LasR ligands. Five novel inducers and three inhibitors of LasR activity were validated experimentally by use of a cell‐based assay. Interestingly, these compounds are molecularly distinct from the native signal molecule, N ‐3‐oxododecanoyl‐ L ‐homoserine lactone (OHN), and may serve as lead structures for the design of new drugs. The binding modes of these compounds to the OHN binding site in LasR were predicted and used to identify the key interactions that contribute to the induction and inhibition of LasR activity.