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Asymmetric Synthesis and Conformational Analysis by NMR Spectroscopy and MD of Aba‐ and α‐MeAba‐Containing Dermorphin Analogues
Author(s) -
Vandormael Bart,
De Wachter Rien,
Martins José C.,
Hendrickx Pieter M. S.,
Keresztes Attila,
Ballet Steven,
Mallareddy Jayapal R.,
Tóth Fanni,
Tóth Géza,
Tourwé Dirk
Publication year - 2011
Publication title -
chemmedchem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.817
H-Index - 100
eISSN - 1860-7187
pISSN - 1860-7179
DOI - 10.1002/cmdc.201100314
Subject(s) - dermorphin , chemistry , stereochemistry , epimer , enantiomer , nuclear magnetic resonance spectroscopy , cyclic peptide , racemization , aminolysis , peptide , catalysis , opioid peptide , biochemistry , opioid , receptor
Dermorphin analogues, containing a ( S )‐ and ( R )‐4‐amino‐1,2,4,5‐tetrahydro‐2‐benzazepin‐3‐one scaffold (Aba) and the α‐methylated analogues as conformationally constrained phenylalanines, were prepared. Asymmetric phase‐transfer catalysis was unable to provide the ( S )‐α‐Me‐ o ‐cyanophenylalanine precursor for ( S )‐α‐MeAba in acceptable enantiomeric purity. However, by using a Schöllkopf chiral auxiliary, this intermediate was obtained in 88 % ee . [( S )‐Aba 3‐Gly 4]dermorphin retained μ‐opioid affinity but displayed an increased δ‐affinity. The corresponding R epimer was considerably less potent. In contrast, the [( R )‐α‐MeAba 3‐Gly 4]dermorphin isomer was more potent than its S epimer. Tar‐MD simulations of both non‐methylated [Aba 3‐Gly 4]dermorphin analogues showed a degree of folding at the C‐terminal residues toward the N terminus of the peptide, without however, adopting a stabilized β‐turn conformation. The α‐methylated analogues, on the other hand, exhibited a type I/I′ β‐turn conformation over the α‐MeAba 3 and Gly 4 residues, which was stabilized by a hydrogen bond involving Tyr 5‐H N and D ‐Ala 2‐CO.