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Cover Picture: A PEG‐Based Biocompatible Block Catiomer with High Buffering Capacity for the Construction of Polyplex Micelles Showing Efficient Gene Transfer toward Primary Cells (ChemMedChem 4/2006)
Author(s) -
Kanayama Naoki,
Fukushima Shigeto,
Nishiyama Nobuhiro,
Itaka Keiji,
Jang WooDong,
Miyata Kanjiro,
Yamasaki Yuichi,
Chung Ungil,
Kataoka Kazunori
Publication year - 2006
Publication title -
chemmedchem
Language(s) - English
Resource type - Reports
SCImago Journal Rank - 0.817
H-Index - 100
eISSN - 1860-7187
pISSN - 1860-7179
DOI - 10.1002/cmdc.200690010
Subject(s) - transfection , micelle , chemistry , ethylenediamine , gene delivery , green fluorescent protein , protonation , biophysics , peg ratio , gene transfer , peptide , dna , cytotoxicity , combinatorial chemistry , gene , biochemistry , in vitro , organic chemistry , ion , biology , finance , aqueous solution , economics
The cover picture shows highly transfection‐efficient and less toxic polyplex micelles formed by the electrostatic interaction between DNA and block catiomers equipped with an ethylenediamine unit at the side chain. The catiomers undergo a conformational transition of the ethylenediamine unit from the gauche (mono‐protonated) to the anti (di‐protonated) form as the pH decreases from physiological to acidic conditions, providing a high buffering capacity for efficient transfection. The polyplex micelles showed effective and non‐cytotoxic transfection of a green fluorescent protein (GFP) gene into mouse primary osteoblast cells (background). For more details, see the full paper by N. Kanayama et al. on p. 439 ff.