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Exploring Metallodrug–Protein Interactions by ESI Mass Spectrometry: The Reaction of Anticancer Platinum Drugs with Horse Heart Cytochrome c
Author(s) -
Casini Angela,
Gabbiani Chiara,
Mastrobuoni Guido,
Messori Luigi,
Moneti Gloriano,
Pieraccini Giuseppe
Publication year - 2006
Publication title -
chemmedchem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.817
H-Index - 100
eISSN - 1860-7187
pISSN - 1860-7179
DOI - 10.1002/cmdc.200500079
Subject(s) - platinum , chemistry , adduct , cytochrome c , combinatorial chemistry , reactivity (psychology) , mass spectrometry , cytochrome , stereochemistry , computational biology , computational chemistry , organic chemistry , catalysis , biochemistry , apoptosis , chromatography , enzyme , medicine , alternative medicine , pathology , biology
Under the right conditions , as shown by high‐quality ESI‐MS spectra, a variety of platinum–protein adducts are formed from the reaction of various platinum drugs with cytochrome c. Met65 is proposed as the primary site for platinum coordination. Direct comparative information was obtained on the reactivity of these classical anticancer platinum compounds with proteins, and the mechanistic and methodological implications of these findings are discussed.