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FLN ‐1/filamin is required to anchor the actomyosin cytoskeleton and for global organization of sub‐cellular organelles in a contractile tissue
Author(s) -
Kelley Charlotte A.,
Triplett Olivia,
Mallick Samyukta,
Burkewitz Kristopher,
Mair William B.,
Cram Erin J.
Publication year - 2020
Publication title -
cytoskeleton
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.95
H-Index - 86
eISSN - 1949-3592
pISSN - 1949-3584
DOI - 10.1002/cm.21633
Subject(s) - filamin , biology , microbiology and biotechnology , myosin , actin , cytoskeleton , microfilament , microtubule , organelle , physarum , endoplasmic reticulum , actin cytoskeleton , cell , physarum polycephalum , biochemistry
Actomyosin networks are organized in space, direction, size, and connectivity to produce coordinated contractions across cells. We use the C. elegans spermatheca, a tube composed of contractile myoepithelial cells, to study how actomyosin structures are organized. FLN‐1/filamin is required for the formation and stabilization of a regular array of parallel, contractile, actomyosin fibers in this tissue. Loss of fln‐1 results in the detachment of actin fibers from the basal surface, which then accumulate along the cell junctions and are stabilized by spectrin. In addition, actin and myosin are captured at the nucleus by the linker of nucleoskeleton and cytoskeleton complex (LINC) complex, where they form large foci. Nuclear positioning and morphology, distribution of the endoplasmic reticulum and the mitochondrial network are also disrupted. These results demonstrate that filamin is required to prevent large actin bundle formation and detachment, to prevent excess nuclear localization of actin and myosin, and to ensure correct positioning of organelles.