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The small molecule AMBMP disrupts microtubule growth, ciliogenesis, cell polarity, and cell migration
Author(s) -
Werner Michael,
del Castillo Urko,
Ventrella Rosa,
Brotslaw Eva,
Mitchell Brian
Publication year - 2018
Publication title -
cytoskeleton
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.95
H-Index - 86
eISSN - 1949-3592
pISSN - 1949-3584
DOI - 10.1002/cm.21496
Subject(s) - ciliogenesis , biology , xenopus , nocodazole , microtubule , cilium , microbiology and biotechnology , regulator , centrosome , cell polarity , polarity (international relations) , cell , cell cycle , genetics , cytoskeleton , gene
2‐Amino‐4‐(3,4‐[methylenedioxy]benzylamino)‐6‐(3‐methoxyphenyl)pyrimidine (AMBMP) is a small molecule that has been previously reported to be both a Wnt agonist and a microtubule (MT) regulator. Here we report a detailed analysis of AMBMPs effects on MTs and on MT associated cellular processes including cell polarity, ciliogenesis, and cell migration. Specifically, treatment of Xenopus embryos with AMBMP leads to defects similar to the MT depolymerizing drug nocodazole, including a failure to generate or polarize cilia (depending on the timing of treatment) and a loss of the cell movements associated with radial intercalation. The dramatic effect AMBMP has on basic MT based cellular functions suggests that its usefulness as a Wnt regulator is questionable. Moreover, it may be an important new tool for experimental or pharmacological manipulation of MTs.