Premium
Flow‐aligned, single‐shot fiber diffraction using a femtosecond X‐ray free‐electron laser
Author(s) -
Popp David,
Loh N. Duane,
Zorgati Habiba,
Ghoshdastider Umesh,
Liow Lu Ting,
Ivanova Magdalena I.,
Larsson Mårten,
DePonte Daniel P.,
Bean Richard,
Beyerlein Kenneth R.,
Gati Cornelius,
Oberthuer Dominik,
Arnlund David,
Brändén Gisela,
Berntsen Peter,
Cascio Duilio,
Chavas Leonard M. G.,
Chen Joe P. J.,
Ding Ke,
Fleckenstein Holger,
Gumprecht Lars,
Harimoorthy Rajiv,
Mossou Estelle,
Sawaya Michael R.,
Brewster Aaron S.,
Hattne Johan,
Sauter Nicholas K.,
Seibert Marvin,
Seuring Carolin,
Stellato Francesco,
Tilp Thomas,
Eisenberg David S.,
Messerschmidt Marc,
Williams Garth J.,
Koglin Jason E.,
Makowski Lee,
Millane Rick P.,
Forsyth Trevor,
Boutet Sébastien,
White Thomas A.,
Barty Anton,
Chapman Henry,
Chen Swaine L.,
Liang Mengning,
Neutze Richard,
Robinson Robert C.
Publication year - 2017
Publication title -
cytoskeleton
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.95
H-Index - 86
eISSN - 1949-3592
pISSN - 1949-3584
DOI - 10.1002/cm.21378
Subject(s) - protein filament , fiber diffraction , femtosecond , diffraction , free electron model , laser , optics , materials science , electron diffraction , fiber , crystallography , biophysics , molecular physics , x ray crystallography , physics , biology , chemistry , composite material
A major goal for X‐ray free‐electron laser (XFEL) based science is to elucidate structures of biological molecules without the need for crystals. Filament systems may provide some of the first single macromolecular structures elucidated by XFEL radiation, since they contain one‐dimensional translational symmetry and thereby occupy the diffraction intensity region between the extremes of crystals and single molecules. Here, we demonstrate flow alignment of as few as 100 filaments ( Escherichia coli pili, F‐actin, and amyloid fibrils), which when intersected by femtosecond X‐ray pulses result in diffraction patterns similar to those obtained from classical fiber diffraction studies. We also determine that F‐actin can be flow‐aligned to a disorientation of approximately 5 degrees. Using this XFEL‐based technique, we determine that gelsolin amyloids are comprised of stacked β‐strands running perpendicular to the filament axis, and that a range of order from fibrillar to crystalline is discernable for individual α‐synuclein amyloids.