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Rapid centriole assembly in N aegleria reveals conserved roles for both de novo and mentored assembly
Author(s) -
FritzLaylin Lillian K.,
Levy Yaron Y.,
Levitan Edward,
Chen Sean,
Cande W. Zacheus,
Lai Elaine Y.,
Fulton Chandler
Publication year - 2016
Publication title -
cytoskeleton
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.95
H-Index - 86
eISSN - 1949-3592
pISSN - 1949-3584
DOI - 10.1002/cm.21284
Subject(s) - centriole , biology , microbiology and biotechnology , organelle , cilium , tetrahymena , eukaryote , mitosis , genetics , genome , gene
Centrioles are eukaryotic organelles whose number and position are critical for cilia formation and mitosis. Many cell types assemble new centrioles next to existing ones (“templated” or mentored assembly). Under certain conditions, centrioles also form without pre‐existing centrioles (de novo). The synchronous differentiation of Naegleria amoebae to flagellates represents a unique opportunity to study centriole assembly, as nearly 100% of the population transitions from having no centrioles to having two within minutes. Here, we find that Naegleria forms its first centriole de novo, immediately followed by mentored assembly of the second. We also find both de novo and mentored assembly distributed among all major eukaryote lineages. We therefore propose that both modes are ancestral and have been conserved because they serve complementary roles, with de novo assembly as the default when no pre‐existing centriole is available, and mentored assembly allowing precise regulation of number, timing, and location of centriole assembly. © 2016 Wiley Periodicals, Inc.