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Computational model of polarized actin cables and cytokinetic actin ring formation in budding yeast
Author(s) -
Tang Haosu,
Bidone Tamara C.,
Vavylonis Dimitrios
Publication year - 2015
Publication title -
cytoskeleton
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.95
H-Index - 86
eISSN - 1949-3592
pISSN - 1949-3584
DOI - 10.1002/cm.21258
Subject(s) - formins , biology , microbiology and biotechnology , actin , actin remodeling , cytokinesis , antiparallel (mathematics) , actin remodeling of neurons , cytoskeleton , myosin , biophysics , mdia1 , microfilament , actin cytoskeleton , cell division , biochemistry , physics , cell , quantum mechanics , magnetic field
The budding yeast actin cables and contractile ring are important for polarized growth and division, revealing basic aspects of cytoskeletal function. To study these formin‐nucleated structures, we built a three‐dimensional (3D) computational model with actin filaments represented as beads connected by springs. Polymerization by formins at the bud tip and bud neck, crosslinking, severing, and myosin pulling, are included. Parameter values were estimated from prior experiments. The model generates actin cable structures and dynamics similar to those of wild type and formin deletion mutant cells. Simulations with increased polymerization rate result in long, wavy cables. Simulated pulling by type V myosin stretches actin cables. Increasing the affinity of actin filaments for the bud neck together with reduced myosin V pulling promotes the formation of a bundle of antiparallel filaments at the bud neck, which we suggest as a model for the assembly of actin filaments to the contractile ring. © 2015 Wiley Periodicals, Inc.