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Actin–myosin network influences morphological response of neuronal cells to altered osmolarity
Author(s) -
Bober Brian G.,
Love James M.,
Horton Steven M.,
Sitnova Mariya,
Shahamatdar Sina,
Kannan Ajay,
Shah Sameer B.
Publication year - 2015
Publication title -
cytoskeleton
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.95
H-Index - 86
eISSN - 1949-3592
pISSN - 1949-3584
DOI - 10.1002/cm.21219
Subject(s) - biology , osmotic concentration , microbiology and biotechnology , cytoskeleton , actin , osmotic shock , osmotic pressure , actin cytoskeleton , myosin , actin remodeling of neurons , biophysics , cell , biochemistry , gene
Acute osmotic fluctuations in the brain occur during a number of clinical conditions and can result in a variety of adverse neurological symptoms. Osmotic perturbation can cause changes in the volumes of intra‐ and extracellular fluid and, due to the rigidity of the skull, can alter intracranial pressure thus making it difficult to analyze purely osmotic effects in vivo . The present study aims to determine the effects of changes in osmolarity on SH‐SY5Y human neuroblastoma cells in vitro, and the role of the actin–myosin network in regulating this response. Cells were exposed to hyper‐ or hypoosmotic media and morphological and cytoskeletal responses were recorded. Hyperosmotic shock resulted in a drop in cell body volume and planar area, a persisting shape deformation, and increases in cellular translocation. Hypoosmotic shock did not significantly alter planar area, but caused a transient increase in cell body volume and an increase in cellular translocation via the development of small protrusions rich in actin. Disruption of the actin–myosin network with latrunculin and blebbistatin resulted in changes to volume and shape regulation, and a decrease in cellular translocation. In both osmotic perturbations, no apparent disruptions to cytoskeletal integrity were observed by light microscopy. Overall, because osmotically induced changes persisted even after volume regulation occurred, it is possible that osmotic stress may play a larger role in neurological dysfunction than currently believed. © 2015 Wiley Periodicals, Inc.